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Nestin Monoclonal antibody, PBS Only

Nestin Monoclonal Antibody for WB,Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB,Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D11H2

Cat No : 66259-1-PBS

Print datasheet

Synonyms

NES, Nestin

验证数据展示

  • WB analysis of Neuro-2a using 66259-1-Ig (same clone as 66259-1-PBS)

    WB analysis of Neuro-2a using 66259-1-Ig (same clone as 66259-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66259-1-Ig (same clone as 66259-1-PBS)

    WB analysis of HEK-293 using 66259-1-Ig (same clone as 66259-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

产品信息

66259-1-PBS targets Nestin in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,Indirect ELISA
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nestin
Synonyms NES, Nestin
Calculated Molecular Weight 177 kDa
Observed Molecular Weight 150-170 kDa
GenBank Accession NumberNM_006617
Gene Symbol NES
Gene ID (NCBI) 10763
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48681
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.