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CoraLite® Plus 488-conjugated Nestin Polyclonal antibody

Nestin Polyclonal Antibody for IF-P,FC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P,FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-19483

Print datasheet

Synonyms

FLJ21841, Nbla00170, NES, nestin

验证数据展示

  • IF Staining of human kidney using CL488-19483

    IF Staining of human kidney using CL488-19483

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Nestin antibody (CL488-19483) at dilution of 1:200.

  • FC experiment of neuronal cells derived from human dental pulp stem using CL488-19483

    FC experiment of neuronal cells derived from human dental pulp stem using CL488-19483

    Neuronal cells derived from human dental pulp stem cells were fixed in 70% ethanol and blocked in 0.1% BSA in Staining Buffer, then stained with CoraLite® Plus 488-conjugated Nestin Polyclonal Antibody (CL488-19483) (1:200) (red) or unstained (blue). Image credit: @stemcell_art on Instagram

经过测试的应用

Positive IF detected inhuman kidney tissue
Positive FC detected inneuronal cells derived from human dental pulp stem cells
For other applications, we recommend the unconjugated version of this antibody, 19483-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19483 targets Nestin in IF, FC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P,FC
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nestin
Synonyms FLJ21841, Nbla00170, NES, nestin
Calculated Molecular Weight 177 kDa
Observed Molecular Weight 170-180 kDa
GenBank Accession NumberNM_006617
Gene Symbol NES
Gene ID (NCBI) 10763
RRIDAB_2919165
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48681
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Nestin antibody CL488-19483Download protocol
Standard Protocols
Click here to view our Standard Protocols