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  • KD/KO Validated

OGT Monoclonal antibody

OGT Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B2A6

Cat No : 66823-1-Ig

Print datasheet

Synonyms

FLJ23071, HRNT1, O GLCNAC, OGT



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy OGT Ready-To-Use IHC Kit. OGT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66823-1-Ig targets OGT in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman, mouse
Immunogen OGT fusion protein Ag28402 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Synonyms FLJ23071, HRNT1, O GLCNAC, OGT
Calculated Molecular Weight 1046 aa, 117 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC014434
Gene Symbol OGT
Gene ID (NCBI) 8473
RRIDAB_2882166
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15294
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.

实验方案

Product Specific Protocols
WB protocol for OGT antibody 66823-1-IgDownload protocol
IHC protocol for OGT antibody 66823-1-IgDownload protocol
IF protocol for OGT antibody 66823-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

FOXA1 O-GlcNAcylation-mediated transcriptional switch governs metastasis capacity in breast cancer

Authors - Yajie Liu
  • KD Validated
humanWB

J Biol Chem

Histone methyltransferase Dot1L recruits O-GlcNAc transferase to target chromatin sites to regulate histone O-GlcNAcylation.

Authors - Bo Xu
human,mouseWB,IHC

Oncogene

Chromatin-associated OGT promotes the malignant progression of hepatocellular carcinoma by activating ZNF263

Authors - Lingyan Wang
  • KD Validated
humanWB,IF

Cell Commun Signal

The deubiquitinase EIF3H promotes hepatocellular carcinoma progression by stabilizing OGT and inhibiting ferroptosis

Authors - Jianing Tang