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OS9 Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 10061-1-AP

Featured Product KD/KO validated

浓度: 130μg/ml

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

OS9 Antibody WB HEK-293 cells 10061-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10061-1-AP(OS9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

OS9 Antibody IHC human lung cancer tissue 10061-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer using 10061-1-AP(OS9 antibody) at dilution of 1:50 (under 10x lens)

OS9 Antibody IF HEK-293 cells 10061-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HEK-293 cells using 10061-1-AP(OS9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human
Positive WB detected in:
HEK-293 cells, HeLa cells
Positive IHC detected in:
human lung cancer tissue, human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HEK-293 cells
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
OS9 fusion protein Ag0106
Full name:
osteosarcoma amplified 9, endoplasmic reticulum associated protein
Calculated molecular weight:
73.4 kDa
Observed molecular weight:
73 kDa
GenBank accession number:
BC000532
Gene ID (NCBI):
10956
Gene symbol
OS9
Synonyms
Amplified in osteosarcoma 9, OS 9, OS9, Protein OS 9

Background

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors. Isoform 2 is the major isoform detected in all cell types examined. This antibody can recognize all three isoforms of OS-9.


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