CoraLite®555-conjugated P53 Recombinant antibody
P53 Recombinant Antibody for FC (Intra), IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
2H13
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 80077-1-PBS
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-80077 targets P53 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | FC (Intra), IF/ICC |
Tested Reactivity | Human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | tumor protein p53 |
Synonyms | Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53 |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 53 kDa |
GenBank Accession Number | BC003596 |
Gene Symbol | TP53 |
Gene ID (NCBI) | 7157 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P04637 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process.
TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)
实验方案
Product Specific Protocols | |
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IF protocol for CL555 P53 antibody CL555-80077 | Download protocol |
FC protocol for CL555 P53 antibody CL555-80077 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |