• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PARP1 Recombinant antibody

PARP1 Recombinant Antibody for IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

IF/ICC,IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3N19

Cat No : 80174-1-RR

Print datasheet

Synonyms

ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL

验证数据展示

  • WB analysis using 80174-1-RR

    WB analysis using 80174-1-RR

    Jurkat cells treated with Staurosporin were subjected to SDS PAGE followed by western blot with 80174-1-RR (PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 80174-1-RR

    IHC staining of human liver cancer using 80174-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 80174-1-RR

    IF Staining of mouse testis using 80174-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 80174-1-RR

    IF Staining of mouse testis using 80174-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse testis using 80174-1-RR

    IHC staining of mouse testis using 80174-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 80174-1-RR

    IF Staining of HepG2 using 80174-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, ROS1728 cells, HepG2 cells, RAW264.7 cells
Positive IHC detected inhuman liver cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue, MCF-7 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

80174-1-RR targets PARP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC,IF-P, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen PARP1 fusion protein Ag4193 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name poly (ADP-ribose) polymerase 1
Synonyms ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL
Calculated Molecular Weight 1014 aa, 113 kDa
Observed Molecular Weight113-116, 89 kDa
GenBank Accession NumberBC037545
Gene Symbol PARP1
Gene ID (NCBI) 142
RRIDAB_2918872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

实验方案

Product Specific Protocols
WB protocol for PARP1 antibody 80174-1-RRDownload protocol
IHC protocol for PARP1 antibody 80174-1-RRDownload protocol
IF protocol for PARP1 antibody 80174-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Macromol

Rosa rugosa polysaccharide induces autophagy-mediated apoptosis in human cervical cancer cells via the PI3K/AKT/mTOR pathway.

Authors - Yue Liu
View Article