验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, HT-1080 cells, HUVEC cells, U-251 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 3 publications below |
产品信息
60045-1-Ig targets PDGFR alpha in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | PDGFR alpha fusion protein Ag1385 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | platelet-derived growth factor receptor, alpha polypeptide |
| Synonyms | CD140A, CD140a antigen, PDGF R alpha, PDGF receptor alpha, PDGFR2, PDGFRA, PDGFRα, PDGFR-α, Rhe PDGFRA |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 100-120 kDa, 140-160 kDa |
| GenBank Accession Number | BC015186 |
| Gene Symbol | PDGFR alpha |
| Gene ID (NCBI) | 5156 |
| RRID | AB_2162343 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P16234 |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival and chemotaxis. Mutations in PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDGFR alpha antibody 60045-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine. | ||
Development The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition | ||
Development The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition. | ||
Metabolism The vitamin D receptor regulates miR-140-5p and targets the MAPK pathway in bone development. |
