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PDI Monoclonal antibody, PBS Only

PDI Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse, rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

2E6A11

Cat No : 66422-1-PBS

Print datasheet

Synonyms

Cell and organelle markers, DSI, endoplasmic reticulum marker, ERBA2L, GIT, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase

验证数据展示

  • IF Staining of HepG2 using 66422-1-Ig (same clone as 66422-1-PBS)

    IF Staining of HepG2 using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDI antibody (66422-1-Ig, Clone: 2E6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • WB analysis using 66422-1-Ig (same clone as 66422-1-PBS)

    WB analysis using 66422-1-Ig (same clone as 66422-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

产品信息

66422-1-PBS targets PDI in WB, IF, IHC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Applications WB,IHC,IF,ELISA
Tested Reactivity human, pig, mouse, rat
Immunogen PDI fusion protein Ag1747 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name prolyl 4-hydroxylase, beta polypeptide
Synonyms Cell and organelle markers, DSI, endoplasmic reticulum marker, ERBA2L, GIT, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC014504
Gene Symbol P4HB
Gene ID (NCBI) 5034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07237
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).