验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, HUVEC cells, NIH/3T3 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10530-1-AP targets PDLIM5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PDLIM5 fusion protein Ag0808 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PDZ and LIM domain 5 |
| Synonyms | PDZ and LIM domain 5, L9, Enigma-like PDZ and LIM domains protein, ENH |
| Calculated Molecular Weight | 64 kDa |
| Observed Molecular Weight | 63-68 kDa |
| GenBank Accession Number | BC008741 |
| Gene Symbol | PDLIM5 |
| Gene ID (NCBI) | 10611 |
| RRID | AB_2161779 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96HC4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDLIM5 (PDZ and LIM domain 5), formerly known as enigma homolog (ENH), is a cytoplasmic protein containing PDZ domain and LIM domain at N terminus and C terminus, respectively. PDLIM5 gene generates several splicing variants, EZH1 to EZH4. The long isoform, ENH1, is widely expressed in various tissues including heart, brain, spleen, liver and kidney. In comparison, shorter isoforms that lack the LIM motifs are expressed in cardiac (ENH3) and skeletal muscle (ENH2, ENH3, and ENH4). ENH1 is essential for differentiation of striated muscles through interaction with other proteins. In addition, PDLIM5 is also involved in mood disorders, such as schizophrenia, bipolar disorder, and major depression. This antibody is specific to PDLIM5, and its specificity has been confirmed by siRNA test.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDLIM5 antibody 10530-1-AP | Download protocol |
| IHC protocol for PDLIM5 antibody 10530-1-AP | Download protocol |
| IP protocol for PDLIM5 antibody 10530-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Differ MicroRNA-17-92 regulates myoblast proliferation and differentiation by targeting the ENH1/Id1 signaling axis.
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Oncotarget High expression of PDLIM5 facilitates cell tumorigenesis and migration by maintaining AMPK activation in prostate cancer.
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Drug Deliv Ultrasound-sensitive siRNA-loaded nanobubbles fabrication and antagonism in drug resistance for NSCLC. | ||
J Mol Cell Cardiol Extracellular vesicular MicroRNA-27a* contributes to cardiac hypertrophy in chronic heart failure.
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Chem Biol Drug Des si-RNA mediated knockdown of PDLIM5 suppresses gastric cancer cell proliferation in vitro.
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Sci Rep Actin cable formation and epidermis-dermis positional relationship during complete skin regeneration |
