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Biotin-conjugated PKM1-specific Polyclonal antibody

PKM1-specific Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Conjugate

Biotin

Cat No : Biotin-15821

Print datasheet

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

验证数据展示

  • IHC staining of mouse brain using Biotin-15821

    IHC staining of mouse brain using Biotin-15821

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-15821(PKM1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using Biotin-15821

    IHC staining of mouse brain using Biotin-15821

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-15821(PKM1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using Biotin-15821

    IHC staining of mouse skeletal muscle using Biotin-15821

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using Biotin-15821 (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse brain tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-15821 targets PKM1-specific in IHC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PKM pyruvate kinase, muscle
Synonyms CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberNM_182470
Gene Symbol PKM
Gene ID (NCBI) 5315
RRIDAB_2883058
Conjugate Biotin
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14618
Storage Buffer PBS with 50% Glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is conjugated with Biotin and it's specific to PKM1 isoform.

实验方案

Product Specific Protocols
IHC protocol for Biotin PKM1-specific antibody Biotin-15821Download protocol
Standard Protocols
Click here to view our Standard Protocols