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tPA Polyclonal antibody 8 Publications

Rabbit Polyclonal| Catalog number: 10147-1-AP

浓度: 330 μg/ml

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Applications

Tested Applications:
IF, IHC, IP, WB, ELISA
Application not tested? Please see our Guarantee


Cited Applications:
IF, IHC, WB


Images See all

IF Staining of mouse brain using 10147-1-AP

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10147-1-AP (tPA antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

WB analysis of mouse pancreas using 10147-1-AP

mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP experiment of mouse kidney using 10147-1-AP

IP Result of anti-tPA (IP:10147-1-AP, 3ug; Detection:10147-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

IHC staining of human pancreas cancer using 10147-1-AP

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10147-1-AP (tPA antibody at dilution of 1:50 (under 10x lens).

IHC staining of human pancreas cancer using 10147-1-AP

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10147-1-AP (tPA antibody at dilution of 1:50 (under 40x lens).

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse pancreas tissue, mouse kidney tissue
Positive IP detected in:
mouse kidney tissue
Positive IHC detected in:
human pancreas cancer tissue, human ovary tumor tissue, human placenta tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
tPA fusion protein Ag0200
Full name:
plasminogen activator, tissue
Calculated molecular weight:
57 kDa
Observed molecular weight:
32-35 kDa, 65 kDa
GenBank accession number:
BC002795
Gene ID (NCBI):
5327
Gene symbol
PLAT
Synonyms
Alteplase, plasminogen activator, tissue, PLAT, Reteplase, T PA, t plasminogen activator, Tissue Plasminogen Activator, tPA

Background

Plasminogen activator, tissue (PLAT, synonyms: TPA, T-PA) is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein (33 kDa and 32 kDa). PLAT enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA has 4 isoforms produced by alternative splicing with the MW of 63 kDa, 33 kDa, 57 kDa and 44 kDa.

Protocols

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