PPAR Gamma Monoclonal antibody

PPAR Gamma Monoclonal Antibody for WB, IHC, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, ELISA, FC (Intra) and More (2)

Conjugate

Unconjugated

CloneNo.

1F4A2

Cat No : 66936-1-Ig

Print datasheet

Synonyms

PPARG, PPARγ, PPAR-gamma, Peroxisome proliferator-activated receptor gamma, NR1C3



经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, mouse adipose tissue, A431 cells, A549 cells, PC-3 cells, K-562 cells, HL-60 cells
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PPARG Ready-To-Use IHC Kit. PPARG primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66936-1-Ig targets PPAR Gamma in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA, FC (Intra) Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen PPAR Gamma fusion protein Ag16657 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name peroxisome proliferator-activated receptor gamma
Synonyms PPARG, PPARγ, PPAR-gamma, Peroxisome proliferator-activated receptor gamma, NR1C3
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC006811
Gene Symbol PPARG
Gene ID (NCBI) 5468
RRIDAB_2882260
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP37231
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, INS resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 INS-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa) (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).

实验方案

Product Specific Protocols
WB protocol for PPAR Gamma antibody 66936-1-IgDownload protocol
IHC protocol for PPAR Gamma antibody 66936-1-IgDownload protocol
FC protocol for PPAR Gamma antibody 66936-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Acta Pharm Sin B

Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1.

Authors - Piao Luo
ratWB

Phytomedicine

Podophyllotoxin via SIRT1/PPAR /NF-κB axis induced cardiac injury in rats based on the toxicological evidence chain (TEC) concept

Authors - Tao Jiang
mouseWB

Front Immunol

miR-10a-3p modulates adiposity and suppresses adipose inflammation through TGF-β1/Smad3 signaling pathway

Authors - Sonia Kiran
humanWB

Clin Transl Med

Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma

Authors - Hao Zhang
mouseWB

Sci Rep

Metformin protects ovarian granulosa cells in chemotherapy-induced premature ovarian failure mice through AMPK/PPAR-γ/SIRT1 pathway

Authors - Yuxin Yang
mouseWB

Nutrients

Exploring Active Ingredients, Beneficial Effects, and Potential Mechanism of Allium tenuissimum L. Flower for Treating T2DM Mice Based on Network Pharmacology and Gut Microbiota

Authors - Shan-Shan Zhang