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验证数据展示

  • WB analysis of MCF-7 using 11658-1-AP

    WB analysis of MCF-7 using 11658-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 11658-1-AP

    IHC staining of human stomach cancer using 11658-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11658-1-AP (CPI17 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • IF Staining of A549 using 11658-1-AP

    IF Staining of A549 using 11658-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CPI17 antibody (11658-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

CPI17 Polyclonal antibody

CPI17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11658-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PPP1R14A, Protein kinase C-potentiated inhibitor protein of 17 kDa, PPP1INL, CPI-17, CPI 17

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells, human colon tissue, Jurkat cells, PC-3 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11658-1-AP targets CPI17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CPI17 fusion protein Ag2237 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 14A
Synonyms PPP1R14A, Protein kinase C-potentiated inhibitor protein of 17 kDa, PPP1INL, CPI-17, CPI 17
Calculated Molecular Weight 147 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC021089
Gene Symbol CPI17
Gene ID (NCBI) 94274
RRIDAB_2252794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96A00
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R14A, also named as CPI17, belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. CPI17 has two isoforms:CPI-17alpha (17 kDa) and CPI-17beta (13 kDa).

实验方案

Product Specific Protocols
WB protocol for CPI17 antibody 11658-1-APDownload protocol
IHC protocol for CPI17 antibody 11658-1-APDownload protocol
IF protocol for CPI17 antibody 11658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols