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Pancreatic Polypeptide Polyclonal antibody

Pancreatic Polypeptide Polyclonal Antibody for FC, IF/ICC,IF-P, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC,IF-P, IHC, ELISA

Conjugate

Unconjugated

Cat No : 15493-1-AP

Print datasheet

Synonyms

Obinepitide, pancreatic polypeptide, Pancreatic prohormone, PH Pancreatic icosapeptide, PI, PNP, PP, PPY

验证数据展示

  • IF Staining of rat pancreas using 15493-1-AP

    IF Staining of rat pancreas using 15493-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat pancreas tissue using Pancreatic Polypeptide antibody (15493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Pancreatic Polypeptide antibody (15493-1-AP, green) stained on PP cells in rat pancreas islet. Glucagon antibody (67286-1-Ig, Clone: 2A8G1, red) stained on α cells in rat pancreas islet.

  • IF Staining of rat pancreas using 15493-1-AP

    IF Staining of rat pancreas using 15493-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat pancreas tissue using Pancreatic Polypeptide antibody (15493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Pancreatic Polypeptide antibody (15493-1-AP) (green) stained on PP cells in rat pancreas islet. Glucagon antibody (67286-1-Ig, Clone: 2A8G1, red) stained on α cells in rat pancreas islet.

  • IHC staining of mouse pancreas using 15493-1-AP

    IHC staining of mouse pancreas using 15493-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15493-1-AP (Pancreatic Polypeptide antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 15493-1-AP

    IHC staining of mouse pancreas using 15493-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15493-1-AP (Pancreatic Polypeptide antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 15493-1-AP

    IF Staining of HepG2 using 15493-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Pancreatic Polypeptide antibody (15493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 15493-1-AP

    FC experiment of HepG2 using 15493-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Pancreatic Polypeptide (15493-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat pancreas tissue, HepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

15493-1-AP targets Pancreatic Polypeptide in IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC,IF-P, IHC, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Pancreatic Polypeptide fusion protein Ag7886 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pancreatic polypeptide
Synonyms Obinepitide, pancreatic polypeptide, Pancreatic prohormone, PH Pancreatic icosapeptide, PI, PNP, PP, PPY
Calculated Molecular Weight 10 kDa
GenBank Accession NumberBC032225
Gene Symbol PPY
Gene ID (NCBI) 5539
RRIDAB_2878145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01298
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Pancreatic Polypeptide antibody 15493-1-APDownload protocol
IF protocol for Pancreatic Polypeptide antibody 15493-1-APDownload protocol
FC protocol for Pancreatic Polypeptide antibody 15493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Transpl Int

Formation of Re-Aggregated Neonatal Porcine Islet Clusters Improves In Vitro Function and Transplantation Outcome

Authors - M Honarpisheh
View Article