CoraLite® Plus 488-conjugated PRC1 Monoclonal antibody
PRC1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E1D5
验证数据展示
经过测试的应用
| Positive IF detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 67027-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67027 targets PRC1 in IF applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IF/ICC |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | PRC1 fusion protein Ag5907 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | protein regulator of cytokinesis 1 |
| Synonyms | ASE1, PRC1 |
| Calculated Molecular Weight | 72 kDa |
| Observed Molecular Weight | 66-72 kDa |
| GenBank Accession Number | BC003138 |
| Gene Symbol | PRC1 |
| Gene ID (NCBI) | 9055 |
| RRID | AB_2919409 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O43663 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PRC1 antibody CL488-67027 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |