Perforin Polyclonal antibody
Perforin Polyclonal Antibody for FC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
FC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | CTLL-2 cells, mouse kidney tissue |
Positive FC detected in | NK-92 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14580-1-AP targets Perforin in WB, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | FC, WB, ELISA |
Cited Applications | WB, IF, FC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, chicken |
Immunogen | Perforin fusion protein Ag6060 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | perforin 1 (pore forming protein) |
Synonyms | Cytolysin, FLH2, HPLH2, P1, Perforin, Perforin 1, PFN1, PFP, PRF1 |
Calculated Molecular Weight | 61 kDa |
Observed Molecular Weight | 70-75 kDa |
GenBank Accession Number | BC063043 |
Gene Symbol | PRF1 |
Gene ID (NCBI) | 5551 |
RRID | AB_10639524 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P14222 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Perforin antibody 14580-1-AP | Download protocol |
FC protocol for Perforin antibody 14580-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Cell Infect Microbiol The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection. | ||
Mol Immunol VEGF165b augments NK92 cytolytic activity against human K562 leukemia cells by upregulating the levels of perforin and granzyme B via the VEGR1-PLC pathway. | ||
Res Vet Sci Characterization of cytotoxicity-related gene expression in response to virulent Marek's disease virus infection in the bursa of Fabricius. | ||
J Exp Clin Cancer Res Targeting MUS81 promotes the anticancer effect of WEE1 inhibitor and immune checkpoint blocking combination therapy via activating cGAS/STING signaling in gastric cancer cells. | ||
Biomed Pharmacother Tannins in Terminalia bellirica inhibits hepatocellular carcinoma growth via re-educating tumor-associated macrophages and restoring CD8+T cell function | ||
J Immunol Res The SIX1/LDHA Axis Promotes Lactate Accumulation and Leads to NK Cell Dysfunction in Pancreatic Cancer |