验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells, HeLa cells, human placenta tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11171-1-AP targets PRPF8 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | PRPF8 fusion protein Ag1652 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) |
| 别名 | PRP8 homolog, PRP8, Pre-mRNA-processing-splicing factor 8, p220, HPRP8 |
| 计算分子量 | 274 kDa |
| 观测分子量 | 220 kDa |
| GenBank蛋白编号 | BC064370 |
| 基因名称 | PRPF8 |
| Gene ID (NCBI) | 10594 |
| RRID | AB_2171179 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6P2Q9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRPF8 antibody 11171-1-AP | Download protocol |
| IHC protocol for PRPF8 antibody 11171-1-AP | Download protocol |
| IP protocol for PRPF8 antibody 11171-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev An integrative analysis of colon cancer identifies an essential function for PRPF6 in tumor growth. | ||
Nucleic Acids Res Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway. | ||
Nucleic Acids Res XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence. | ||
J Biol Chem Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly. |
