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PRTN3 Monoclonal antibody

PRTN3 Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D1B8

Cat No : 67030-1-Ig

Print datasheet

Synonyms

ACPA, AGP7, C ANCA, C ANCA antigen, Leukocyte proteinase 3, MBN, MBT, Myeloblastin, Neutrophil proteinase 4, P29, PR 3, PR3, proteinase 3, PRTN3, Wegener autoantigen

验证数据展示

  • WB analysis of human placenta using 67030-1-Ig

    WB analysis of human placenta using 67030-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 67030-1-Ig

    IHC staining of human spleen using 67030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 67030-1-Ig

    IHC staining of human spleen using 67030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 67030-1-Ig

    IF Staining of human tonsillitis using 67030-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PRTN3 antibody (67030-1-Ig, Clone: 2D1B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67030-1-Ig targets PRTN3 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity Human
Immunogen PRTN3 fusion protein Ag25915 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name proteinase 3
Synonyms ACPA, AGP7, C ANCA, C ANCA antigen, Leukocyte proteinase 3, MBN, MBT, Myeloblastin, Neutrophil proteinase 4, P29, PR 3, PR3, proteinase 3, PRTN3, Wegener autoantigen
Calculated Molecular Weight 256 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC096183
Gene Symbol PRTN3
Gene ID (NCBI) 5657
RRIDAB_2882345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24158
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRTN3 antibody 67030-1-IgDownload protocol
IHC protocol for PRTN3 antibody 67030-1-IgDownload protocol
IF protocol for PRTN3 antibody 67030-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols