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CoraLite®594-conjugated PTBP1 Monoclonal antibody

PTBP1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D6D8

Cat No : CL594-67462

Print datasheet

Synonyms

HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1

验证数据展示

  • IF Staining of A375 using CL594-67462

    IF Staining of A375 using CL594-67462

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using CoraLite®594 PTBP1 antibody (CL594-67462, Clone: 3D6D8 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-67462

    FC experiment of HeLa using CL594-67462

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PTBP1 (CL594-67462, Clone:3D6D8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inA375 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PTBP1 Ready-To-Use IHC Kit. PTBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67462-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67462 targets PTBP1 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen PTBP1 fusion protein Ag28404 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name polypyrimidine tract binding protein 1
Synonyms HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC013694
Gene Symbol PTBP1
Gene ID (NCBI) 5725
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP26599
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10.

实验方案

Product Specific Protocols
IF protocol for CL594 PTBP1 antibody CL594-67462Download protocol
FC protocol for CL594 PTBP1 antibody CL594-67462Download protocol
Standard Protocols
Click here to view our Standard Protocols