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PTEN Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 10047-1-AP

Featured Product KD/KO validated

浓度: 130μg/ml

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

PTEN Antibody WB MCF-7 cells 10047-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

PTEN Antibody WB HeLa cells 10047-1-AP

WB result of PTEN antibody (10047-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTEN transfected HeLa cells

PTEN Antibody IHC human prostate cancer tissue 10047-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 10047-1-AP( PTEN Antibody) at dilution of 1:50

PTEN Antibody IHC human prostate cancer tissue 10047-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 10047-1-AP( PTEN Antibody) at dilution of 1:50

Species specificity:
human, mouse
Positive WB detected in:
MCF-7 cells, HeLa cells, DU 145 cells
Positive IHC detected in:
human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PTEN fusion protein Ag0007
Full name:
phosphatase and tensin homolog
Calculated molecular weight:
47 kDa
Observed molecular weight:
55 kDa
GenBank accession number:
BC005821
Gene ID (NCBI):
5728
Gene symbol
PTEN
Synonyms
10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

Background

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.


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