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PTEN Polyclonal antibody 2 Publications

Rabbit Polyclonal| Catalog number: 10047-1-AP

Featured Product KD/KO validated

浓度: 300 μg/ml

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Applications

Tested Applications:
IHC, WB,ELISA
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Cited Applications:
IHC, WB


Images See all

WB analysis of HeLa using 10047-1-AP

WB result of PTEN antibody (10047-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTEN transfected HeLa cells.

WB analysis using 10047-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

IHC staining of human breast cancer using 10047-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10047-1-AP (PTEN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IHC staining of human breast cancer using 10047-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10047-1-AP (PTEN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Species specificity:
human, mouse
Positive WB detected in:
MCF-7 cells, HeLa cells, DU 145 cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PTEN fusion protein Ag0007
Full name:
phosphatase and tensin homolog
Calculated molecular weight:
47 kDa
Observed molecular weight:
55 kDa
GenBank accession number:
BC005821
Gene ID (NCBI):
5728
Gene symbol
PTEN
Synonyms
10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

Background

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.

Protocols

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