Name: PTEN Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IHC, ELISA
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Various lysates were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

WB result of PTEN antibody (10047-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTEN transfected HeLa cells

PTEN Antibody IHC human prostate cancer tissue 10047-1-AP

Immunohistochemistry of paraffin-embedded human prostate cancer slide using 10047-1-AP( PTEN Antibody) at dilution of 1:50

Species specificity:

human, mouse

Positive WB detected in:

MCF-7 cells, HeLa cells, DU 145 cells

Positive IHC detected in:

human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution:

WB : 1:500-1:1000

IHC : 1:50-1:500

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

PTEN fusion protein Ag0007

Full name:

phosphatase and tensin homolog

Calculated molecular weight:

47 kDa

Observed molecular weight:

55 kDa

GenBank accession number:

BC005821

Gene ID (NCBI):

5728

Gene symbol

PTEN

Synonyms

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

Background

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.

Showing 1- [2] of [2] Publications

Author:		Jiong Lyu
Pubmed ID:		26577500
Journal:		Oral Surg Oral Med Oral Pathol Oral Radiol
Title:		Predictive value of pAKT/PTEN expression in oral squamous cell carcinoma treated with cetuximab-based chemotherapy.
Application:		IHC

Species:		human

Author:		Tao Zeng
Pubmed ID:		28987868
Journal:		Redox Biol
Title:		Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.
Application:		WB

Species:		human

WB Figures

HeLa cells were subjected to SDS PAGE followed by western blot with 10047-1-AP(PTEN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours