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Phospho-mTOR (Ser2481) Polyclonal antibody

Phospho-mTOR (Ser2481) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 28879-1-AP

Print datasheet

Synonyms

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2481), RAFT1, Rapamycin target protein 1, RAPT1

验证数据展示

  • WB analysis using 28879-1-AP

    WB analysis using 28879-1-AP

    Nontreated and insulin-treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28879-1-AP (Phospho-mTOR (Ser2481) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inInsulin treated NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

28879-1-AP targets Phospho-mTOR (Ser2481) in WB, ELISA applications and shows reactivity with Mouse samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity Mouse
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FK506 binding protein 12-rapamycin associated protein 1
Synonyms FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2481), RAFT1, Rapamycin target protein 1, RAPT1
Calculated Molecular Weight 289 kDa
Observed Molecular Weight250-289 kDa
GenBank Accession NumberBC117166
Gene Symbol FRAP1
Gene ID (NCBI) 2475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42345
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTOR, also named FRAP1, FRAP, FRAP2, and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is the kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. mTORC2 is also activated by growth factors but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.

实验方案

Product Specific Protocols
WB protocol for Phospho-mTOR (Ser2481) antibody 28879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Ethnopharmacol

Iridoids from Gardeniae fructus ameliorates depression by enhancing synaptic plasticity via AMPA receptor-mTOR signaling

Authors - Baomei Xia
View Article
mouseWB

Chem Biol Interact

Oral administration of sophoricoside (SOP) inhibits neuronal damage and neuroinflammation to curb neurodegeneration in Parkinson's disease

Authors - Hefei Wang
View Article