Gamma Catenin Monoclonal antibody
Gamma Catenin Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, pig, Canine, mouse
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1A5D10
验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, A431 cells, HT-29 cells, MDCK cells, rat heart tissue, T-47D cells |
| Positive IHC detected in | human breast cancer tissue, human skin tissue, human stomach cancer tissue, mouse stomach tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A431 cells, MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy JUP Ready-To-Use IHC Kit. JUP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
66445-1-Ig targets Gamma Catenin in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, pig, Canine, mouse samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, rat, pig, Canine, mouse |
| Cited Reactivity | human |
| Immunogen | Gamma Catenin fusion protein Ag1627 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | junction plakoglobin |
| Synonyms | ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 82 kDa |
| GenBank Accession Number | BC011865 |
| Gene Symbol | JUP |
| Gene ID (NCBI) | 3728 |
| RRID | AB_2881814 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P14923 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Gamma Catenin antibody 66445-1-Ig | Download protocol |
| IHC protocol for Gamma Catenin antibody 66445-1-Ig | Download protocol |
| IF protocol for Gamma Catenin antibody 66445-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) DSC2 suppresses the growth of gastric cancer through the inhibition of nuclear translocation of γ-catenin and PTEN/PI3K/AKT signaling pathway | ||
Oxid Med Cell Longev DSC2 Suppresses the Metastasis of Gastric Cancer through Inhibiting the BRD4/Snail Signaling Pathway and the Transcriptional Activity of β-Catenin |
