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RABEPK/p40 Polyclonal antibody

RABEPK/p40 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15105-1-AP

Print datasheet

Synonyms

40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK, RABEPK/p40

验证数据展示

  • WB analysis of HepG2 using 15105-1-AP

    WB analysis of HepG2 using 15105-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 15105-1-AP

    IHC staining of human lung cancer using 15105-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 15105-1-AP

    IHC staining of human lung cancer using 15105-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 15105-1-AP

    IP experiment of HeLa using 15105-1-AP

    IP result of anti-RABEPK/p40 (IP:15105-1-AP, 4ug; Detection:15105-1-AP 1:1000) with HeLa cells lysate 2080 ug.

  • IF Staining of HeLa using 15105-1-AP

    IF Staining of HeLa using 15105-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RABEPK/p40 antibody (15105-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 15105-1-AP

    FC experiment of HeLa using 15105-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human RABEPK/p40 (15105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, K-562 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15105-1-AP targets RABEPK/p40 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity human
Immunogen RABEPK/p40 fusion protein Ag2593 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rab9 effector protein with kelch motifs
Synonyms 40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK, RABEPK/p40
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC065725
Gene Symbol RABEPK
Gene ID (NCBI) 10244
RRIDAB_1557623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6M1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

实验方案

Product Specific Protocols
WB protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
IHC protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
IF protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
IP protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
FC protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols