RABEPK/p40 Monoclonal antibody
RABEPK/p40 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1E11A3
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HEK-293 cells, HSCT6 cells, RAW 264.7 cells |
| Positive IHC detected in | human lung cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A549 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
66622-1-Ig targets RABEPK/p40 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | IHC |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | RABEPK/p40 fusion protein Ag7796 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Rab9 effector protein with kelch motifs |
| Synonyms | 40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK, RABEPK/p40 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC065725 |
| Gene Symbol | RABEPK |
| Gene ID (NCBI) | 10244 |
| RRID | AB_2881982 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q7Z6M1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RABEPK/p40 antibody 66622-1-Ig | Download protocol |
| IHC protocol for RABEPK/p40 antibody 66622-1-Ig | Download protocol |
| IF protocol for RABEPK/p40 antibody 66622-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Exp Ther Med Management of a rare ovarian carcinosarcoma: A case report and literature review |
