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CoraLite® Plus 488-conjugated RGS4 Monoclonal antibody

RGS4 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E2E2

Cat No : CL488-66441

Print datasheet

Synonyms

DKFZp761F1924, RGP4, RGS4, SCZD9

验证数据展示

  • WB analysis using CL488-66441

    WB analysis using CL488-66441

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66441 (RGS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using CL488-66441

    WB analysis using CL488-66441

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66441 (RGS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inC6 cells, Neuro-2a cells, Raji cells, SH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 66441-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66441 targets RGS4 in WB applications and shows reactivity with human, rat, mouse, pig samples.

Tested Applications WB
Tested Reactivity human, rat, mouse, pig
Immunogen RGS4 fusion protein Ag6029 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name regulator of G-protein signaling 4
Synonyms DKFZp761F1924, RGP4, RGS4, SCZD9
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC051869
Gene Symbol RGS4
Gene ID (NCBI) 5999
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49798
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Regulator of G protein signaling (RGS) proteins are a large family of highly diverse, multifunctional signaling proteins. RGS4 is one of seven members of a classic R4 RGS protein that accelerates the GTPase activity of the Gai/o and Gaq/11 family members. RGS4 is highly expressed in multiple brain regions including the cerebral cortex, amygdala, hippocampus, and striatum. Extensive studies have demonstrated that RGS4 plays an important role in regulating smooth muscle contraction, cardiomyocyte development, neural plasticity and psychiatric disorders.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 RGS4 antibody CL488-66441Download protocol
Standard Protocols
Click here to view our Standard Protocols