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RNF125 Polyclonal antibody

RNF125 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 13290-1-AP

Print datasheet

Synonyms

ring finger protein 125, RNF125, TRAC 1, TRAC1

验证数据展示

  • WB analysis of mouse spleen using 13290-1-AP

    WB analysis of mouse spleen using 13290-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13290-1-AP (RNF125 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 13290-1-AP

    IHC staining of human lymphoma using 13290-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13290-1-AP (RNF125 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lymphoma using 13290-1-AP

    IHC staining of human lymphoma using 13290-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13290-1-AP (RNF125 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse spleen tissue, A2780 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

RNF125 expresses at a very low level in most cells and tissues.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13290-1-AP targets RNF125 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RNF125 fusion protein Ag3908 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 125
Synonyms ring finger protein 125, RNF125, TRAC 1, TRAC1
Calculated Molecular Weight 232 aa, 26 kDa
Observed Molecular Weight 22-26 kDa
GenBank Accession NumberBC012021
Gene Symbol RNF125
Gene ID (NCBI) 54941
RRIDAB_10597852
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF125 antibody 13290-1-APDownload protocol
IHC protocol for RNF125 antibody 13290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

Type I interferon/STAT1 signaling regulates UBE2M-mediated antiviral innate immunity in a negative feedback manner

Authors - Xianghui Kong
View Article
humanWB,IHC

Oncogene

RNF125 attenuates hepatocellular carcinoma progression by downregulating SRSF1-ERK pathway

Authors - Zhigang Feng
  • KD Validated
View Article