RNF34 Polyclonal antibody
RNF34 Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10629-1-AP targets RNF34 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | RNF34 fusion protein Ag0982 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ring finger protein 34 |
| Synonyms | CARP 1, Caspase regulator CARP1, FYVE RING finger protein Momo, hRFI, RFI, RIF, RIFF, ring finger protein 34, RING finger protein RIFF, RNF34 |
| Calculated Molecular Weight | 42 kDa |
| GenBank Accession Number | BC007826 |
| Gene Symbol | RNF34 |
| Gene ID (NCBI) | 80196 |
| RRID | AB_2301135 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969K3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF34 is an E3 ubiquitin ligase that regulates protein substrates by post-translational modification. It is associated with regulating postsynaptic γ2-GABAAR clustering and GABAergic synaptic innervation in hippocampal neurons, indicating the importance of RNF34 in regulating neurological function.It is preferentially expressed in esophageal, gastric, and colorectal cancers, suggesting a possible association between RNF34 and the development of digestive tract cancers. Immunoblots showed a single 42-kDa protein band corresponding to the molecular weight of RNF34.(PMID: 31704983, PMID: 25193658)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RNF34 antibody 10629-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun A ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells
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