ROCK1 Monoclonal antibody
ROCK1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3G5E12
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
66782-1-Ig targets ROCK1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Immunogen | ROCK1 fusion protein Ag16317 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Rho-associated, coiled-coil containing protein kinase 1 |
| Synonyms | p160 ROCK 1, P160ROCK, PRO0435, ROCK1 |
| Calculated Molecular Weight | 1354 aa, 158 kDa |
| Observed Molecular Weight | 150-160 kDa |
| GenBank Accession Number | BC113114 |
| Gene Symbol | ROCK1 |
| Gene ID (NCBI) | 6093 |
| RRID | AB_2882127 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13464 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ROCK1 antibody 66782-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biochem Lnc-MUC20-9 binds to ROCK1 and functions as a tumor suppressor in bladder cancer. | ||
Am J Transl Res β-catenin regulates myocardial ischemia/reperfusion injury following heterotopic heart transplantation in mice by modulating PTEN pathways. | ||
Food Funct Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology | ||
Gene LINC00645 inhibits renal cell carcinoma progression by interacting with HNRNPA2B1 to regulate the ROCK1 mRNA stability |