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RPLP0 Monoclonal antibody

RPLP0 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A2E1

Cat No : 68056-1-Ig

Print datasheet

Synonyms

60S ribosomal protein L10E, L10E, P0, PRLP0, ribosomal protein, large, P0, RPLP0, RPP0

验证数据展示

  • WB analysis using 68056-1-Ig

    WB analysis using 68056-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68056-1-Ig (RPLP0 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 68056-1-Ig

    IHC staining of human colon cancer using 68056-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68056-1-Ig (RPLP0 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPLP0 antibody included

经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, A549 cells, A375 cells, A431 cells, NCI-H1299 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RPLP0 Ready-To-Use IHC Kit. RPLP0 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68056-1-Ig targets RPLP0 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, Mouse , Rat
Cited Reactivityrat
Immunogen RPLP0 fusion protein Ag1829 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribosomal protein, large, P0
Synonyms 60S ribosomal protein L10E, L10E, P0, PRLP0, ribosomal protein, large, P0, RPLP0, RPP0
Calculated Molecular Weight 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC009867
Gene Symbol RPLP0
Gene ID (NCBI) 6175
RRIDAB_2918797
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05388
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPLP0 antibody 68056-1-IgDownload protocol
IHC protocol for RPLP0 antibody 68056-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
rat

Biomolecules

Dietary Soy Prevents Alcohol-Mediated Neurocognitive Dysfunction and Associated Impairments in Brain Insulin Pathway Signaling in an Adolescent Rat Model.

Authors - Ming Tong
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