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验证数据展示

  • WB analysis using 16680-1-AP

    WB analysis using 16680-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16680-1-AP (RPS13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16680-1-AP

    IF Staining of HeLa using 16680-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16680-1-AP (RPS13 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 16680-1-AP

    IHC staining of human colon cancer using 16680-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16680-1-AP (RPS13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPS13 antibody included

RPS13 Polyclonal antibody

RPS13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16680-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Small ribosomal subunit protein uS15, ribosomal protein S13, 40S ribosomal protein S13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, mouse oterus tissue, rat oterus tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPS13 Ready-To-Use IHC Kit. RPS13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

16680-1-AP targets RPS13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RPS13 fusion protein Ag10008 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S13
别名 Small ribosomal subunit protein uS15, ribosomal protein S13, 40S ribosomal protein S13
计算分子量 17 kDa
观测分子量 14 kDa, 17 kDa
GenBank蛋白编号BC006772
基因名称 RPS13
Gene ID (NCBI) 6207
RRIDAB_2182500
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62277
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS13, also named as 40S ribosomal protein S13, belongs to the ribosomal protein S15P family. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. RPS13 is located in the cytoplasm. It is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

实验方案

Product Specific Protocols
WB protocol for RPS13 antibody 16680-1-APDownload protocol
IHC protocol for RPS13 antibody 16680-1-APDownload protocol
IF protocol for RPS13 antibody 16680-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell

Circadian clocks are modulated by compartmentalized oscillating translation

Authors - Yanrong Zhuang
View Article
humanWB

Stem Cells

Genome-wide studies reveal that Lin28 enhances the translation of genes important for growth and survival of human embryonic stem cells.

Authors - Peng Shuping S
View Article
humanWB

N/A

N/A

Authors - N/A
View Article
WB

Cell

An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes

Authors - Keely Oltion
View Article