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CoraLite® Plus 488-conjugated RUVBL2 Monoclonal antibody

RUVBL2 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A1A4

Cat No : CL488-67851

Print datasheet

Synonyms

48 kDa TBP interacting protein, CGI 46, ECP 51, ECP51, INO80 complex subunit J, INO80J, Repressing pontin 52, REPTIN, Reptin 52, RuvB like 2, RuvB like 2 (E. coli), RUVBL2, RVB2, TAP54 beta, TIH2, TIP48, TIP49B

验证数据展示

  • IF Staining of HepG2 using CL488-67851

    IF Staining of HepG2 using CL488-67851

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 RUVBL2 antibody (CL488-67851, Clone: 1A1A4 ) at dilution of 1:200.

  • FC experiment of HL-60 using CL488-67851

    FC experiment of HL-60 using CL488-67851

    1X10^6 HL-60 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RUVBL2 (CL488-67851, Clone:1A1A4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHL-60 cells
Planning an IHC experiment? We recommend our IHCeasy RUVBL2 Ready-To-Use IHC Kit. RUVBL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67851-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67851 targets RUVBL2 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen RUVBL2 fusion protein Ag0253 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name RuvB-like 2 (E. coli)
Synonyms 48 kDa TBP interacting protein, CGI 46, ECP 51, ECP51, INO80 complex subunit J, INO80J, Repressing pontin 52, REPTIN, Reptin 52, RuvB like 2, RuvB like 2 (E. coli), RUVBL2, RVB2, TAP54 beta, TIH2, TIP48, TIP49B
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC000428
Gene Symbol RUVBL2
Gene ID (NCBI) 10856
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y230
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RUVBL2 belongs to the conserved ATPases associated with various cellular activities (AAA+) protein subfamily, which is characterized by the presence of conserved Walker A and B motifs that are involved in ATP binding and hydrolysis. The AAA+ (ATPases associated with diverse cellular activities) ATPases, RUVBL1 and RUVBL2, were originally isolated as components of transcriptional complexes and shown to function in a number of different cellular processes, including transcriptional regulation, chromatin remodelling and DNA damage responses. (PMID: 31572066, PMID: 31138842)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RUVBL2 antibody CL488-67851Download protocol
FC protocol for CL Plus 488 RUVBL2 antibody CL488-67851Download protocol
Standard Protocols
Click here to view our Standard Protocols