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CoraLite® Plus 488-conjugated Recoverin Polyclonal antibody

Recoverin Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10073

Print datasheet

Synonyms

Protein CAR, RCV1, RCVRN, recoverin

验证数据展示

  • IF Staining of mouse eye using CL488-10073

    IF Staining of mouse eye using CL488-10073

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CoraLite® Plus 488 Recoverin antibody (CL488-10073) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF detected inmouse eye tissue
For other applications, we recommend the unconjugated version of this antibody, 10073-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10073 targets Recoverin in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P
Tested Reactivity human, mouse, rat
Immunogen Recoverin fusion protein Ag0119 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name recoverin
Synonyms Protein CAR, RCV1, RCVRN, recoverin
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC001720
Gene Symbol RCVRN
Gene ID (NCBI) 5957
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35243
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Recoverin, belonging to a family of the neuronal calcium sensor (NCS) proteins, has a restricted expression in retinal photoreceptors or neurons or neuroendocrine cells. It has been suggested to play a role in light and dark adaptation by regulating rhodopsin phosphorylation. Recently, it has been found that autoantibodies against recoverin (24 kDa) have been strongly associated with cancer -associated retinopathy (CAR) syndrome, a paraneoplastic disease of the retina. But functions of recoverin in cancer cells remain unknown.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Recoverin antibody CL488-10073Download protocol
Standard Protocols
Click here to view our Standard Protocols