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验证数据展示

  • WB analysis using 66293-1-Ig

    WB analysis using 66293-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66293-1-Ig (SETDB1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 66293-1-Ig

    IHC staining of mouse colon using 66293-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SETDB1 antibody included
  • IHC staining of human colon using 66293-1-Ig

    IHC staining of human colon using 66293-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SETDB1 antibody included
  • IF Staining of A431 using 66293-1-Ig

    IF Staining of A431 using 66293-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SETDB1 antibody (66293-1-Ig, Clone: 1H6E5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

SETDB1 Monoclonal antibody

SETDB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66293-1-Ig

产品说明书

CloneNo. 1H6E5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain, EC:2.1.1.366

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, human heart tissue, MCF-7 cells, Jurkat cells, HepG2 cells
Positive IHC detected inhuman colon tissue, rat liver tissue, rat colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SETDB1 Ready-To-Use IHC Kit. SETDB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66293-1-Ig targets SETDB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SETDB1 fusion protein Ag21644 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SET domain, bifurcated 1
别名 Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain, EC:2.1.1.366
计算分子量 143 kDa
观测分子量 170-180 kDa
GenBank蛋白编号BC009362
基因名称 SETDB1
Gene ID (NCBI) 9869
RRIDAB_2881676
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15047
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).

实验方案

Product Specific Protocols
WB protocol for SETDB1 antibody 66293-1-IgDownload protocol
IHC protocol for SETDB1 antibody 66293-1-IgDownload protocol
IF protocol for SETDB1 antibody 66293-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols