• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

SF3A3 Polyclonal antibody

SF3A3 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12070-1-AP

Print datasheet

Synonyms

PRP9, PRPF9, SAP 61, SAP61, SF3A3, SF3a60, Splicing factor 3A subunit 3

验证数据展示

  • WB analysis using 12070-1-AP

    WB analysis using 12070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12070-1-AP

    IHC staining of human lung cancer using 12070-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12070-1-AP (SF3A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SF3A3 antibody included
  • IP experiment of Raji using 12070-1-AP

    IP experiment of Raji using 12070-1-AP

    IP result of anti-SF3A3 (IP:12070-1-AP, 4ug; Detection:12070-1-AP 1:600) with Raji cells lysate 2000ug.

  • IF Staining of HeLa using 12070-1-AP

    IF Staining of HeLa using 12070-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12070-1-AP (SF3A3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of A549 using 12070-1-AP

    WB analysis of A549 using 12070-1-AP

    WB result of SF3A3 antibody (12070-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SF3A3 transfected A549 cells.

经过测试的应用

Positive WB detected inA431 cells, Raji cells, A549 cells, K-562 cells, NIH/3T3 cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SF3A3 Ready-To-Use IHC Kit. SF3A3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12070-1-AP targets SF3A3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitybovine
Immunogen SF3A3 fusion protein Ag2708 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor 3a, subunit 3, 60kDa
Synonyms PRP9, PRPF9, SAP 61, SAP61, SF3A3, SF3a60, Splicing factor 3A subunit 3
Calculated Molecular Weight 501 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC011523
Gene Symbol SF3A3
Gene ID (NCBI) 10946
RRIDAB_2186372
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SF3A3 antibody 12070-1-APDownload protocol
IHC protocol for SF3A3 antibody 12070-1-APDownload protocol
IF protocol for SF3A3 antibody 12070-1-APDownload protocol
IP protocol for SF3A3 antibody 12070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
bovineWB

Int J Mol Sci

Autophagy Mediates Escherichia Coli-Induced Cellular Inflammatory Injury by Regulating Calcium Mobilization, Mitochondrial Dysfunction, and Endoplasmic Reticulum Stress

Authors - Jianguo Liu
View Article