SF3B3 Polyclonal antibody
SF3B3 Polyclonal Antibody for IF/ICC,IF-P, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IF/ICC,IF-P, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, mouse heart tissue, human brain tissue, rat brain tissue, rat heart tissue, HeLa cells |
Positive IP detected in | rat brain tissue |
Positive IHC detected in | human lung cancer tissue, human gliomas tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells, mouse heart tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14577-1-AP targets SF3B3 in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC,IF-P, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, RIP, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | SF3B3 fusion protein Ag5980 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | splicing factor 3b, subunit 3, 130kDa |
Synonyms | KIAA0017, RSE1, SAP 130, SAP130, SF3b130, SF3B3, Splicing factor 3B subunit 3, STAF130 |
Calculated Molecular Weight | 136 kDa |
Observed Molecular Weight | 130-135 kDa |
GenBank Accession Number | BC003146 |
Gene Symbol | SF3B3 |
Gene ID (NCBI) | 23450 |
RRID | AB_2270189 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q15393 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for SF3B3 antibody 14577-1-AP | Download protocol |
IHC protocol for SF3B3 antibody 14577-1-AP | Download protocol |
IF protocol for SF3B3 antibody 14577-1-AP | Download protocol |
IP protocol for SF3B3 antibody 14577-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Gastroenterology Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p. | ||
Nat Commun Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex. | ||
Nucleic Acids Res Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''. | ||
Mol Neurodegener Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing. | ||
Nucleic Acids Res Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation. |