14-3-3 sigma Antibody 66251-1-Ig

Tested Applications

Applications:

ELISA, IF, IHC, WB
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Various cell lysates were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human skin tissue slide using 66251-1-Ig( SFN Antibody) at dilution of 1:2000 (under 10x lens).

Immunohistochemistry of paraffin-embedded human skin tissue slide using 66251-1-Ig( SFN Antibody) at dilution of 1:2000 (under 40x lens).

Immunofluorescent analysis of MCF-7 cells using 66251-1-Ig( SFN Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:

human

Positive WB detected in:

A431, MCF-7, HeLa cells, HeLa cells

Positive IHC detected in:

human skin tissue, human breast hyperplasia tissue, human skin tissue

Positive IF detected in:

MCF-7 cells

Recommended dilution:

WB : 1:2000-1:14000

IHC : 1:200-1:8000

IF : 1:20-1:200

Product Information

Source:

Mouse

Purification method:

Protein G purification

Isotype:

IgG1

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

14-3-3 sigma fusion protein Ag12644

Full name:

stratifin

Calculated molecular weight:

28 kDa

Observed molecular weight:

28-30 kDa

GenBank accession number:

BC002995

Gene ID (NCBI):

2810

Gene symbol

SFN

Synonyms

14 3 3 protein sigma, HME1, SFN, stratifin, YWHAS

Background

14-3-3 sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.

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WB Figures

A431 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig( SFN Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

Various cell lysates were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig( SFN Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IHC Figures

66251-1-Ig;human breast hyperplasia tissue

Immunohistochemistry of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig( SFN Antibody) at dilution of 1:2000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig( SFN Antibody) at dilution of 1:2000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).