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14-3-3 Sigma Monoclonal antibody 1 Publications

Mouse Monoclonal| Catalog number: 66251-1-Ig |CloneNo.: 3F10D9

浓度: 1373 μg/ml

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Applications

Tested Applications:
FC, IF, IHC, WB,ELISA
Application not tested? Please see our Guarantee


Cited Applications:
IF


Images See all

WB analysis using 66251-1-Ig

Various cell lysates were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

IHC staining of human skin using 66251-1-Ig

Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens).

IHC staining of human skin using 66251-1-Ig

Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens).

IF Staining of MCF-7 using 66251-1-Ig

Immunofluorescent analysis of MCF-7 cells using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

FC experiment of A431 using 66251-1-Ig

1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human 14-3-3 Sigma (66251-1-Ig, Clone:3F10D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Samples were fixed with 90% MeOH.

Species specificity:
human, mouse
Positive WB detected in:
A431 cells, HeLa cells, MCF-7 cells
Positive IHC detected in:
human skin tissue, human breast cancer tissue, human breast hyperplasia tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Positive FC detected in:
A431 cells
Recommended dilution:
WB : 1:2000-1:14000
IHC : 1:200-1:8000
IF : 1:20-1:200

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
14-3-3 Sigma fusion protein Ag12644
Full name:
stratifin
Calculated molecular weight:
28 kDa
Observed molecular weight:
28-30 kDa
GenBank accession number:
BC002995
Gene ID (NCBI):
2810
Gene symbol
SFN
Synonyms
14 3 3 protein sigma, HME1, SFN, stratifin, YWHAS

Background

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.

Protocols

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