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SIRT3 Polyclonal antibody

SIRT3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29838-1-AP

Print datasheet

Synonyms

hSIRT3, SIR2 like protein 3, SIR2L3, SIRT3

验证数据展示

  • WB analysis using 29838-1-AP

    WB analysis using 29838-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29838-1-AP (SIRT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 29838-1-AP

    IHC staining of human prostate cancer using 29838-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29838-1-AP (SIRT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 29838-1-AP

    IHC staining of human prostate cancer using 29838-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29838-1-AP (SIRT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29838-1-AP targets SIRT3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen SIRT3 fusion protein Ag31333 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
Synonyms hSIRT3, SIR2 like protein 3, SIR2L3, SIRT3
Calculated Molecular Weight 44 kDa
Observed Molecular Weight28-30 kDa
GenBank Accession NumberBC001042
Gene Symbol SIRT3
Gene ID (NCBI) 23410
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NTG7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIRT3 antibody 29838-1-APDownload protocol
IHC protocol for SIRT3 antibody 29838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols