SIX2 Antibody 92 Publications

Rabbit Polyclonal| Catalog number: 11562-1-AP

Featured Product KD/KO validated

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Species specificity:
human, mouse, rat, American alligator

Positive WB detected in:
HEK-293 cells, HEK-293 cells, L02 cells, PC-3 cells

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human kidney tissue, human kidney tissue, human renal cell carcinoma tissue

Positive IF detected in:
mouse embryonic kidney, heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice

ApplicationNote:
"The Six2 antibody works great and is really specific to metanephricmesenchyme (kidney progenitor cells). We used it for IF and we highlyrecommend it!" Dr. Aleksandra Rak-Raszewska, University of Liverpool

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:100
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
SIX homeobox 2

Calculated molecular weight:
291aa,32 kDa

Observed molecular weight:
32-37 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
SIX2

Synonyms
Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2
Background

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.


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