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SIX2 Polyclonal antibody

SIX2 Polyclonal Antibody for IF, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

IF, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 11562-1-AP

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Synonyms

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2



经过测试的应用

Positive IP detected inHEK-293 cells
Positive IF detected inmouse embryonic kidney, heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11562-1-AP targets SIX2 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IP, ELISA
Cited Applications ChIP, IP, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat, rabbit, pig, Alligator, canine
Immunogen SIX2 fusion protein Ag2124 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SIX homeobox 2
Synonyms Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight 32-37 kDa
GenBank Accession NumberBC024033
Gene Symbol SIX2
Gene ID (NCBI) 10736
RRIDAB_2189084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPC8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.

实验方案

Product Specific Protocols
WB protocol for SIX2 antibody 11562-1-APDownload protocol
IP protocol for SIX2 antibody 11562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Nature

Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.

Authors - Minoru Takasato
human

Nat Biotechnol

Tubuloids derived from human adult kidney and urine for personalized disease modeling.

Authors - Frans Schutgens
human

Nat Biotechnol

Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D.

Authors - Ravian L van Ineveld
mouse

Cell

In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.

Authors - Hsin-Kai Liao
human

Cell

Tridimensional Visualization and Analysis of Early Human Development.

Authors - Morgane Belle
mouse

Cell Stem Cell

Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells.

Authors - Atsuhiro Taguchi