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验证数据展示

  • WB analysis using 28055-1-AP

    WB analysis using 28055-1-AP

    Boiled and unboiled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 28055-1-AP (SLC12A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28055-1-AP

    IHC staining of human breast cancer using 28055-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28055-1-AP (SLC12A2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 28055-1-AP

    IHC staining of human breast cancer using 28055-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28055-1-AP (SLC12A2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 28055-1-AP

    IF Staining of HeLa using 28055-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NKCC1,SLC12A2 antibody (28055-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 28055-1-AP

    IHC staining of human colon cancer using 28055-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 28055-1-AP (NKCC1,SLC12A2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NKCC1,SLC12A2 Polyclonal antibody

NKCC1,SLC12A2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28055-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

NKCC1, SLC12A2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC2, BSC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inunboiled HeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

For optimal WB detection with 28055-1-AP, we do not recommend boiling the sample after lysis.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28055-1-AP targets NKCC1,SLC12A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 NKCC1,SLC12A2 fusion protein Ag27895 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 12 (sodium/potassium/chloride transporters), member 2
别名 NKCC1, SLC12A2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC2, BSC
计算分子量 131 kDa
观测分子量150-160 kDa
GenBank蛋白编号BC033003
基因名称 NKCC1/SLC12A2
Gene ID (NCBI) 6558
RRIDAB_2881048
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55011
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

实验方案

Product Specific Protocols
WB protocol for NKCC1,SLC12A2 antibody 28055-1-APDownload protocol
IHC protocol for NKCC1,SLC12A2 antibody 28055-1-APDownload protocol
IF protocol for NKCC1,SLC12A2 antibody 28055-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols