验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, MOLT-4 cells, PC-12 cells |
| Positive IHC detected in | human lung cancer tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10420-1-AP targets SLC25A3 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Immunogen | SLC25A3 fusion protein Ag0667 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3 |
| Synonyms | PiC, PTP, Phosphate transport protein, PHC, OK/SW-cl.48 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40-43 kDa |
| GenBank Accession Number | BC003504 |
| Gene Symbol | SLC25A3 |
| Gene ID (NCBI) | 5250 |
| RRID | AB_2877735 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00325 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mitochondrial phosphate carrier SLC25A3 belongs to the SLC25 family , which is the largest family of solute carriers. SLC25A3 encodes the phosphate carrier PiC that catalyses the import of phosphate into mitochondria where it is needed for ATP synthesis. SLC25A3 has two isoforms SLC25A3-A (a heart-type isoform) and SLC25A3-B (a liver-type isoform) as a result of alternative splicing of exon 3. It has been reported that SLC25A3 mutations are associated with mitochondrial phosphate carrier deficiency, respiratory distress and hypertrophic cardiomyopathy. (PMID: 15984930, 27780865, 20877624)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC25A3 antibody 10420-1-AP | Download protocol |
| IHC protocol for SLC25A3 antibody 10420-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Respir Cell Mol Biol Transcriptomics Analysis Identifies the Decline in the AT2 Stem Cell Niche in Aged Human Lungs | ||
Heliyon Prognostic value and immunological function of cuproptosis-related genes in lung adenocarcinoma | ||
Exp Gerontol METTL14-mediated methylation of SLC25A3 mitigates mitochondrial damage in osteoblasts, leading to the improvement of osteoporosis
| ||
Dev Cell Slc25a3-dependent copper transport controls flickering-induced Opa1 processing for mitochondrial safeguard |
