验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, MOLT-4 cells, PC-12 cells |
| Positive IHC detected in | human lung cancer tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10420-1-AP targets SLC25A3 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SLC25A3 fusion protein Ag0667 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3 |
| 别名 | PiC, PTP, Phosphate transport protein, PHC, OK/SW-cl.48 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 40-43 kDa |
| GenBank蛋白编号 | BC003504 |
| 基因名称 | SLC25A3 |
| Gene ID (NCBI) | 5250 |
| RRID | AB_2877735 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q00325 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mitochondrial phosphate carrier SLC25A3 belongs to the SLC25 family , which is the largest family of solute carriers. SLC25A3 encodes the phosphate carrier PiC that catalyses the import of phosphate into mitochondria where it is needed for ATP synthesis. SLC25A3 has two isoforms SLC25A3-A (a heart-type isoform) and SLC25A3-B (a liver-type isoform) as a result of alternative splicing of exon 3. It has been reported that SLC25A3 mutations are associated with mitochondrial phosphate carrier deficiency, respiratory distress and hypertrophic cardiomyopathy. (PMID: 15984930, 27780865, 20877624)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC25A3 antibody 10420-1-AP | Download protocol |
| IHC protocol for SLC25A3 antibody 10420-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Respir Cell Mol Biol Transcriptomics Analysis Identifies the Decline in the AT2 Stem Cell Niche in Aged Human Lungs | ||
Heliyon Prognostic value and immunological function of cuproptosis-related genes in lung adenocarcinoma | ||
Exp Gerontol METTL14-mediated methylation of SLC25A3 mitigates mitochondrial damage in osteoblasts, leading to the improvement of osteoporosis
| ||
Dev Cell Slc25a3-dependent copper transport controls flickering-induced Opa1 processing for mitochondrial safeguard |
