验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse liver tissue, human colon tissue, human heart tissue, SMMC-7721 cells, mouse colon tissue, rat colon tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11013-1-AP targets FATP4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | FATP4 fusion protein Ag1490 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 27 (fatty acid transporter), member 4 |
| 别名 | ACSVL4, FATP 4, FATP4, Fatty acid transport protein 4, SLC27A4 |
| 计算分子量 | 72 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC009959 |
| 基因名称 | FATP4/SLC27A4 |
| Gene ID (NCBI) | 10999 |
| RRID | AB_2190637 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6P1M0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FATP4 antibody 11013-1-AP | Download protocol |
| IHC protocol for FATP4 antibody 11013-1-AP | Download protocol |
| IP protocol for FATP4 antibody 11013-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol Cathelicidin-WA Facilitated Intestinal Fatty Acid Absorption Through Enhancing PPAR-γ Dependent Barrier Function. | ||
iScience Celastrol inhibits intestinal lipid absorption by reprofiling the gut microbiota to attenuate high-fat diet-induced obesity. | ||
Int J Mol Sci Nano-Zn Increased Zn Accumulation and Triglyceride Content by Up-Regulating Lipogenesis in Freshwater Teleost, Yellow Catfish Pelteobagrus fulvidraco. | ||
Biochim Biophys Acta Uninephrectomy augments the effects of high fat diet induced obesity on gene expression in mouse kidney. | ||
Cells LPS Inhibits Fatty Acid Absorption in Enterocytes through TNF-α Secreted by Macrophages. |
