SMAD3 Monoclonal antibody

SMAD3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA and More (3)

Conjugate

Unconjugated

CloneNo.

1F10B2

Cat No : 66516-1-Ig

Print datasheet

Synonyms

hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3



经过测试的应用

Positive WB detected inHT-1080 cells, NIH/3T3 cells, A431 cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66516-1-Ig targets SMAD3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA
Cited Applications CoIP, IF, IHC, WB
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SMAD family member 3
Synonyms hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberNM_005902
Gene Symbol SMAD3
Gene ID (NCBI) 4088
RRIDAB_2881879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP84022
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

实验方案

Product Specific Protocols
WB protocol for SMAD3 antibody 66516-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui
humanWB

Drug Des Devel Ther

Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling

Authors - Huan Hu
human,mouseWB

Cell Chem Biol

Substrate-dependent interaction of SPOP and RACK1 aggravates cardiac fibrosis following myocardial infarction

Authors - Wanqi Yang
mouseWB,IF

Cell Mol Life Sci

Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion

Authors - Shiju Ye
ratWB

Oxid Med Cell Longev

Smad7 Is Highly Expressed in Human Degenerative Discs and Participates in IL-1β-Induced Apoptosis of Rat AF Cells via the Mitochondria Pathway.

Authors - Bo Li
mouseWB

Mol Ther Nucleic Acids

Genetic or siRNA inhibition of MBD2 attenuates the UUO- and I/R-induced renal fibrosis via downregulation of EGR1.

Authors - Kai Ai