CoraLite®594-conjugated SMARCA4 Monoclonal antibody
SMARCA4 Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2E6B6
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 66561-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66561 targets SMARCA4 in IF, FC (Intra) applications and shows reactivity with Human samples.
| Tested Applications | FC (Intra), IF/ICC |
| Tested Reactivity | Human |
| Immunogen | SMARCA4 fusion protein Ag16256 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 |
| Synonyms | ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, FLJ39786, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1 |
| Calculated Molecular Weight | 1647 aa, 185 kDa |
| GenBank Accession Number | BC150298 |
| Gene Symbol | SMARCA4 |
| Gene ID (NCBI) | 6597 |
| RRID | AB_2883603 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P51532 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. And this anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 SMARCA4 antibody CL594-66561 | Download protocol |
| FC protocol for CL594 SMARCA4 antibody CL594-66561 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
