验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, LNCaP cells, MCF-7 cells, RAW 264.7 cells, HEK-293 cells, HepG2 cells, Jurkat cells, SK-BR-3 cells, IMR-32 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SRF Ready-To-Use IHC Kit. SRF primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
66742-1-Ig targets SRF in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SRF fusion protein Ag10386 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | serum response factor (c-fos serum response element-binding transcription factor) |
| 别名 | Serum response factor, MCM1, 1G8F4 |
| 计算分子量 | 508 aa, 52 kDa |
| GenBank蛋白编号 | BC048211 |
| 基因名称 | SRF |
| Gene ID (NCBI) | 6722 |
| RRID | AB_2882090 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11831 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SRF antibody 66742-1-Ig | Download protocol |
| IF protocol for SRF antibody 66742-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis | ||
bioRxiv Single molecule imaging of the central dogma reveals myosin-2A gene expression is regulated by contextual translational buffering
| ||
Mol Neurobiol SRF Facilitates Transcriptional Inhibition of Gem Expression by m6A Methyltransferase METTL3 to Suppress Neuronal Damage in Epilepsy |
