STAT6 70kDa Polyclonal antibody

STAT6 70kDa Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10257-1-AP

Print datasheet

Synonyms

D12S1644, IL 4 STAT, STAT6, STAT6 70kDa, STAT6B, STAT6C



经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STAT6 Ready-To-Use IHC Kit. STAT6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 10257-1-AP is STAT6 70kDa Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen STAT6 70kDa fusion protein Ag0410 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal transducer and activator of transcription 6, interleukin-4 induced
Synonyms D12S1644, IL 4 STAT, STAT6, STAT6 70kDa, STAT6B, STAT6C
Calculated Molecular Weight 100 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC005823
Gene Symbol STAT6
Gene ID (NCBI) 6778
RRIDAB_10695044
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42226
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. This poly-antibody only recognizes 70 kDa isoform of STAT6.

实验方案

Product Specific Protocols
WB protocol for STAT6 70kDa antibody 10257-1-APDownload protocol
IHC protocol for STAT6 70kDa antibody 10257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Isovitexin Inhibits Ginkgolic Acids-Induced Inflammation Through Downregulating SHP2 Activation.

Authors - Yiwei Zhang
ratWB

Pharm Biol

Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF.

Authors - Ying Xu