验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, SH-SY5Y cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10586-1-AP targets STMN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken, zebrafish |
| 免疫原 | STMN2 fusion protein Ag0929 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | stathmin-like 2 |
| 别名 | SGC10, SGC 10, SCGN10, SCGN 10, SCG10 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 18-21 kDa |
| GenBank蛋白编号 | BC006302 |
| 基因名称 | STMN2 |
| Gene ID (NCBI) | 11075 |
| RRID | AB_2197283 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q93045 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STMN2 antibody 10586-1-AP | Download protocol |
| IHC protocol for STMN2 antibody 10586-1-AP | Download protocol |
| IF protocol for STMN2 antibody 10586-1-AP | Download protocol |
| IP protocol for STMN2 antibody 10586-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Molecular substrates of altered axonal growth and brain connectivity in a mouse model of schizophrenia. | ||
Proc Natl Acad Sci U S A SCG10 is a JNK target in the axonal degeneration pathway.
| ||
J Cell Biol SARM1 acts downstream of neuroinflammatory and necroptotic signaling to induce axon degeneration. | ||
Cell Rep Comparative transcriptomic profiling reveals a role for Olig1 in promoting axon regeneration | ||
J Cell Biol A neuroprotective agent that inactivates prodegenerative TrkA and preserves mitochondria. | ||
J Cell Biol Actomyosin-II protects axons from degeneration induced by mild mechanical stress |
