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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated STRAP Monoclonal antibody

STRAP Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5E11F3

Cat No : CL594-66712

Print datasheet

Synonyms

MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD

验证数据展示

  • IF Staining of HEK-293 using CL594-66712

    IF Staining of HEK-293 using CL594-66712

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 STRAP antibody (CL594-66712, Clone: 5E11F3 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy STRAP Ready-To-Use IHC Kit. STRAP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66712-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66712 targets STRAP in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen STRAP fusion protein Ag13085 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serine/threonine kinase receptor associated protein
Synonyms MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC000162
Gene Symbol STRAP
Gene ID (NCBI) 11171
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y3F4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.

实验方案

Product Specific Protocols
IF protocol for CL594 STRAP antibody CL594-66712Download protocol
Standard Protocols
Click here to view our Standard Protocols