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STX17 Recombinant antibody

STX17 Recombinant Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4G3

Cat No : 81899-1-RR

Print datasheet

Synonyms

FLJ20651, STX17, syntaxin 17

验证数据展示

  • WB analysis using 81899-1-RR

    WB analysis using 81899-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81899-1-RR (STX17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 81899-1-RR

    IF Staining of HepG2 using 81899-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using STX17 antibody (81899-1-RR, Clone: 4G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman testis tissue, A375 cells, HEK-293 cells, HepG2 cells, L02 cells, mouse testis issue, rat testis tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81899-1-RR targets STX17 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen STX17 fusion protein Ag12134 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name syntaxin 17
Synonyms FLJ20651, STX17, syntaxin 17
Calculated Molecular Weight 302 aa, 33 kDa
Observed Molecular Weight 33-35 kD
GenBank Accession NumberBC111009
Gene Symbol STX17
Gene ID (NCBI) 55014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56962
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. Syntaxin 17 (STX17) is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. STX17 may also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi.

实验方案

Product Specific Protocols
WB protocol for STX17 antibody 81899-1-RRDownload protocol
IF protocol for STX17 antibody 81899-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols