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SUZ12 Polyclonal antibody
SUZ12 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20366-1-AP targets SUZ12 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IHC, CoIP |
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | suppressor of zeste 12 homolog (Drosophila) |
| Synonyms | ChET 9 protein, CHET9, JJAZ1, Joined to JAZF1 protein, KIAA0160, Polycomb protein SUZ12, SUZ12 |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 80-90 kDa |
| GenBank Accession Number | NM_015355 |
| Gene Symbol | SUZ12 |
| Gene ID (NCBI) | 23512 |
| RRID | AB_10694152 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15022 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SUZ12 antibody 20366-1-AP | Download protocol |
| IHC protocol for SUZ12 antibody 20366-1-AP | Download protocol |
| IP protocol for SUZ12 antibody 20366-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell TRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways.
| ||
EMBO J A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination. | ||
Eur J Med Chem Discovery of precision targeting EZH2 degraders for triple-negative breast cancer. | ||
Sci Rep Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1. | ||
Am J Cancer Res N-terminal truncated carboxypeptidase E represses E-cadherin expression in lung cancer by stabilizing the Snail-HDAC complex. | ||
Acta Pharmacol Sin AZD9291 inactivates the PRC2 complex to mediate tumor growth inhibition. |
