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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Septin 9 Polyclonal antibody

Septin 9 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-10769

Print datasheet

Synonyms

AF17q25, FLJ75490, KIAA0991, MLL septin like fusion protein, MSF, MSF1, NAPB, Ov/Br septin, Ovarian/Breast septin, PNUTL4, SEPT9, SeptD1, septin 9, Septin D1, SINT1

验证数据展示

  • IF Staining of HeLa using CL647-10769

    IF Staining of HeLa using CL647-10769

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 647 Septin 9 antibody (CL647-10769) at dilution of 1:200.

  • FC experiment of HepG2 using CL647-10769

    FC experiment of HepG2 using CL647-10769

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Septin 9 (CL647-10769) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 10769-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10769 targets Septin 9 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen Septin 9 fusion protein Ag1218 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name septin 9
Synonyms AF17q25, FLJ75490, KIAA0991, MLL septin like fusion protein, MSF, MSF1, NAPB, Ov/Br septin, Ovarian/Breast septin, PNUTL4, SEPT9, SeptD1, septin 9, Septin D1, SINT1
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65 kDa, 38-40 kDa
GenBank Accession NumberBC021192
Gene Symbol SEPT9
Gene ID (NCBI) 10801
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHD8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC). this antibody can identify all of the isoforms about SEPT9.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Septin 9 antibody CL647-10769Download protocol
FC protocol for CL Plus 647 Septin 9 antibody CL647-10769Download protocol
Standard Protocols
Click here to view our Standard Protocols